Biopharma application solutions
Connect with Masterflex® for single-use simplicity and the convenience of our industry-leading expertise, comprehensive product offerings and custom assemblies for complete application workflows.
Bioprocessing applications rely on Masterflex®
Masterflex offers the widest selection of trusted brands in the bioprocess market for tubing, fittings, gaskets, sanitary fittings and clamps and more. Too much to choose from? Consult with our technical experts to help you get exactly what you need. Whatever your process needs — filtration, fluid lines, process containment, process control, cell generation, or sampling — you can count on Masterflex to supply the exact component you need to improve your efficiency.
Media prep is a vital step in the upstream process in which the solution for cell culture is prepared. It requires significant time and effort with innovative approaches, including the use of single-use technology, throughout the entire process. Each approach is individually configured based on the manufacturers’ application. Peristaltic pumps play an important role in dosing the exact amounts of process nutrients and other media using high purity, flexible tubing. Peristaltic pumps can also gently recirculate the cells in the solution and protect cell viability.
Industrial fermentation involves sampling a tank of controlled pH, aeration, agitation and temperature. It requires more preliminary steps than other bioprocess workflows. Common microorganisms used in this process are Chinese hamster ovary cells (CHO), yeast, fungi and bacteria. Masterflex® peristaltic pumps are useful in this workflow and are recommended over diaphragm pumps. Peristaltic pumps provide accurate dosing capabilities and, unlike diaphragm pumps, have an isolated and unconvoluted fluid path. This makes maintenance easy and ensures no product gets stuck in a crevice.
Typical harvest methods include tangential flow filtration (TFF) or centrifugation as a means of concentrating the target compound. In either of these methods, quality and purity are paramount. Wetted materials (any material touching the fluid) must be validated to USP Class VI and sterilized. Peristaltic pumps remain accurate at higher pressures, making them ideal for filtration centric workflows.
Affinity and ion-exchange chromatography are used to remove metals and reaction by-products along with additional methods of filtration. Recently, single-use tube component assemblies have become more prevalent in this workflow as higher throughputs and versatility on the process floor are demanded. The isolated fluid flow path of peristaltic pumps, such as Masterflex® pumps, is critical to this workflow. These pumps mitigate the potential for contamination and reduce the required material validation.
Fill-Finish: Gently transfer sterile product for packaging or lyophilization
This critical process — highly regulated with global regulatory and compliance requirements — transfers the sterile product, now in its most valuable form, to a sterile vial or bottle before it is packaged and distributed or sent to lyophilization (freeze-drying) before packaging. Peristaltic pumps are the top choice because of their ability to provide easy changeovers, reduce cross-contamination and gently pump shear-sensitive fluids, large molecules and live cells. The valuable end product is contained in the tubing, never touching any part of the pump. Our single-use sterilized assemblies, developed specifically for this process, eliminate downtime for cleaning.
Trusted configurations that meet the highest quality in assembly and sterilization.
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Masterflex single-use advantage - managing the quality and risk of your single-use systems.
More than 100 formulations, provided in bulk, bagged, or packaged per your requirements.
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Our experts will help you design your custom bioprocess assembly with the brands, components and validation you need.